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Image Search Results
Journal: British Journal of Pharmacology
Article Title: Distinct effects of CGRP on typical and atypical smooth muscle cells involved in generating spontaneous contractions in the mouse renal pelvis
doi: 10.1111/j.1476-5381.2009.00514.x
Figure Lengend Snippet: Distribution of PGP9.5-, calcitonin gene-related peptide (CGRP)- and transient receptor potential cation channel, subfamily V, member 1 (TRPV1) immunoreactive nerves in the mouse renal pelvis. In the proximal renal pelvis, nerve fibres were stained with antibodies against the neural marker PGP 9.5 (Aa). CGRP-immunoreactive nerve fibres were abundant in the same preparation (Ab). A merged image indicates that most of thick and thin nerve bundles contain CGRP-immunoreactive fibres (Ac). Double staining of the mid renal pelvis with TRPV1 (Ba) and CGRP (Bb) antibodies showed that many CGRP-positive fibres also expressed TRPV1-immnoreactivity (Bc). Scale bars: 80 µm.
Article Snippet: To examine colocalization of CGRP and transient receptor potential cation channels (TRPV1; vanilloid receptors), whole mounts were incubated with
Techniques: Staining, Marker, Double Staining